Figure 0

Description

• AIRE interacting network.
  AIRE (autoimmune regulator protein, depicted with the green circle) regulates central tolerance in thymic medullary epithelial cells. This protein localizes in small dots (green staining) through the nucleus (red staining). To get insights into the molecular mechanism by which AIRE regulates gene expression, AIRE interactors (yellow circles) were isolated and identified by quantitative proteomic approach (immunoprecipitation of SILAC labelled cells). Bioinformatics tools revealed the biological functions involved in AIRE activity.
• Have you ever wonder how sea urchins attach to the rocks and withstand the force of waves? The answer is a powerful bioadhesive secreted by specialized adhesive organs. Part of this bioadhesive is made of proteins that can be separated in a gel and then extracted for subsequent identification and characterization. Unlike common adhesives these bioadhesives are non-toxic, biodegradable and efficient in the presence of water, which makes them excellent candidates for the development of future biomedical or industrial glues.
• Have you ever wonder how sea urchins attach to the rocks and withstand the force of waves? The answer is a powerful bioadhesive secreted by specialized adhesive organs. Part of this bioadhesive is made of proteins that can be separated in a gel and then extracted for subsequent identification and characterization. Unlike common adhesives these bioadhesives are non-toxic, biodegradable and efficient in the presence of water, which makes them excellent candidates for the development of future biomedical or industrial glues.
• Eight repeated images obtained by Western Blot technique were compiled to form this picture. Westerns are a powerful method to pin-point specific proteins that are separated by size on a gel. The gel is transferred to a membrane and in this case gel was ripped before transference and pieces were replaced in an attempt to reconstitute the original one. To my perception it generated an image that resembles birds flying and I named it: 'birds heading north'.
• In Gel Shooting Stars 17 hours of Fame
  Proteins breaking through the gel matrix, speeding across time and space, making all together tracks resembling shooting stars. The author wants to convey proteomics of the restricted group of scientists who develop this scientific branch of biology, to the scientific culture in general, provoking in the observer an idea of mass distribution of the proteomics knowledge. To this end the author used the ideas of Andy Warhol in which the icon chosen to be 'warholise' was a two-dimensional electrophoresis gel.
• Is-it the twilight of 'gel-based proteomics' ? In the last ten years, 'mass spectrometry based' proteomic has shown a great potential in proteomic studies, with a lot of conferences in the subject. But it is mainly reserved for labs and proteomic platforms with large financial capacities. It is very important to highlight the capacities of gel-based proteomics in order to encourage doing such 'low-cost' experiments, which is emphasised with most of biological questions.
• Do you know what you're drinking? Or are you attracted by extraordinary advertising like this poster of Armando Testa? The beauty of woman reflects the refreshing taste of Blue Ribbon beer. As for the first question: by using combinatorial peptide ligand libraries (CPLL, pH 7 and 9 capture), we make visible the invisible proteome, namely twenty residual barley proteins and 40 unique gene products from Saccharomyces cerevisiae present in traces, not visible in the control. Let us drink to that!
• Two layer distance field visualization of protein identifications from a specifically isolated interactome analyzed by high accuracy mass spectrometry. The bottom layer comprises of roughly 50 proteins each represented by a circle. The identification score is graphed against the molecular mass and the diameter of one circle correlates with the quality of the assignment. A more stringent method was applied to identify the proteins represented in the top layer.
• Freedom
  In her work the author wants to make an abstract representation of a singular technique that makes possible visualised in vivo proteins. The collage presents spiral of repetitions from a microscopic image of a red blood cell, where is possible to visualize in green the presence and localization of the protein cytochrome-b5 reductase, studied in the rare disease "Hereditary Methemoglobinemia". The title "Freedom" makes allusion to the self-government gain from the absence of homeostasis, when disease happens.
• Molecular image of sperm transit in the rat epididymis by MALDI imaging mass spectrometry. In mammals, sperm maturation occurs during epididymal transit. Peptides specific to maturing spermatozoa or epididymal ducts morphology can be visualized simultaneously. Mass signal overlay of three specific peptides of 5470, 6177 and 18746 Da is visualized in different colors. A 80 µm lateral image resolution is mandatory to resolve the organ structures.
• ARTWORK: Differential Proteomics: Under the spot lights of DIGE.
• ARTWORK: Marine organisms as a source of new molecules and animal models to be studied by Proteomics.
• ARTWORK: Why use sea stars as a model to study regeneration? Why not ??? Echinoderms are the closest living relative to chordates placing them in a favorable position as model organisms! Their amazing capability of regeneration is an interesting case study for proteomics approaches. In this artwork an image of a 2D gel of the nervous system (represented in orange) of the sea star that derives from the circumoral nerve ring.
• This image pretends to compile a study which aim was to apply quantitative proteomics based on 2D-DIGE to get clues into the molecular mechanisms underlying the adaptive strategies employed by Burkholderia cenocepacia to deal with the stressing conditions of the cystic fibrosis (CF) lung including antibiotic therapy. The expression programs of two clonal isolates retrieved from a chronically infected CF patient and exhibiting a different susceptibility profile towards a wide range of antimicrobials were compared.
• This image pretends to compile a study which aim was to apply quantitative proteomics based on 2D-DIGE to get clues into the molecular mechanisms underlying the adaptive strategies employed by Burkholderia cenocepacia to deal with the stressing conditions of the cystic fibrosis (CF) lung including antibiotic therapy. The expression programs of two clonal isolates retrieved from a chronically infected CF patient and exhibiting a different susceptibility profile towards a wide range of antimicrobials were compared.
• Cells in our bodies communicate with each other through a complex process called signalling. To study the hundreds of proteins that create these signals, we captured them in a jelly-like gel and stained them with a dye. The gels have been distorted to resemble citrus fruits (to encourage a juicy discovery!). The bands on the gels are the hundreds of signalling proteins captured when a cell communicates. Signalling really is complex!
• Cells in our bodies communicate with each other through a complex process called signalling. Cells send and receive signals using antennae-like receptors on their outer membranes. We developed a technique to look at the hundreds of proteins that create these signals. The image shows a reconstructed map of how signalling proteins may interact when a cell communicates. The receptors are the rings at the bottom left, and each circle represents an extra potential level of signalling. Signalling really is complex!
• Cells in our bodies communicate with each other through a complex process called signalling. Cells send and receive signals using antennae-like receptors on their outer membranes. We developed a technique to look at the hundreds of proteins that create these signals. The image shows some of the first ever views of the unique "barcode" of specific signalling proteins in a cell. The "heat" of each tiny bar represents the amount of that protein (red, large). Signalling really is complex!
• Cells in our bodies communicate with each other through a complex process called signalling. Cells send and receive signals using antennae-like receptors on their outer membranes. We developed a technique to look at the hundreds of proteins that create these signals. The image shows the unique "barcode" of signalling proteins in a cell (yellow). Part of this barcode has been reconstructed as a network to show how the proteins may interact when a cell communicates. Signalling really is complex!
• A researcher strayed in the protein forest. Species are flowering around and their striking diversity amazes and hampers the proceeding along the way at the same time. But despite the difficulties of the path, the hope is ahead, and it's necessary to proceed.
• The eye of a researcher and proteins of the visual system: rhodopsin, arrestin, recoverin, retinitis pigmentosa 2 protein, guanylate cyclase, cataract causing mutant of human gammaD crystalline and others. The answers for questions of life are inside the inquirer, and proteomics serves a mirror to unveil them.

Technical description

• Fluorescence was visualized with an inverted fluorescence microscope and captured with confocal laser microscope (Leica). Protein network analysis was done using IntAct (www.ebi.ac.uk/intact/) database system and analysis tools for protein interaction data to retrieve the known interactors of the identified proteins and the results were visualized with Cytoscape 2.7.0.
• Nikon Coolpix 990 (1-D electrophoresis system and sea urchin); GE Healthcare ImageScanner II (1D gel); Applied Biosystems MALDI-TOF/TOF 4800 plus mass spectrometer (m/z spectra).
• Nikon Coolpix 990 (seashore); JEOL JSM-6100 transmission electron microscope (sea urchin adhesive organ); Leitz Orthoplan light microscope and Leica DC 300F digital camera (bioadhesive footprint); GE Healthcare IPGphor system; Ettan Dalt 6 and ImageScanner II (2D gel).
• Proteins, separated by electrophoresis, were transferred to a membrane and exposed to serum from a mouse with a malaria-like infection. Secondary antibody horseradish peroxidase-labeled was used to develop reaction. Images were acquired in 'Kodak Image Station' using 'Molecular Imaging Software'.
• Gel scan 300dpi, picture manipulated (colour&light) in Microsoft Office Picture Manager, repetitions mounted in Microsoft Paint. Isoelectric focusing=100V0.5hG,100V3.0hS,200V3.0hS,1000V6.0hS, 3000V4.0hS,6000V2.0G,8000V2.0hG,8000V2.0hS; SDS-PAGE=2.0h100V, 17h1000V; silver coloration. Protein from methanol precipitation of phenolic solution resulted from liver's RNA extraction.
• 100 µg of total protein extracts from roots of Arabidopsis thaliana were focused on pI 4-7 range and separated on gels covering the 15-150 kDa range. Proteins spots were visualized by colloidal Coomassie blue staining.
• Picture of Armando Testa taken with a Panasonic DMC-FZ5 camera, with an optical zoom of 12X and Leica lens. 2D maps images acquired by VersaDoc Imaging System Model 3000 (Bio-Rad), with a 3.2 megapixel CCD ( 53 mm resolution).
• The multi channel alpha blending distance field visualization was performed on a NVIDIA GeForce 8800 Ultra graphic board in real time at the Interactive Graphics Systems Group at TU Darmstadt.
• Microscopic image taken from confocal instrument, red blood cells analysed by Immunofluorescence technique with anti-cytochrome-b5 reductase.
• Image acquired on a MALDI molecular imager (Autoflex III Smartbeam) at 80 µm lateral resolution using the FlexImaging software (Bruker Daltonics).
• The 2D gel image is from the nervous tissue of a sea star and was taken in a regular Gel Scanner and the 3D appearance and the light effects were performed using 3D motion graphics software (Autodesk Maya 2011).
• The 2D gel image is from a the nerve cord of a sea star and was taken in a regular Gel Scanner and the 3D marine organisms were modeled using 3D motion graphics software (Autodesk Maya 2011).
• The 2D gel image of the nerve cord was taken in a regular Gel Scanner and the 3D representation of the sea star was modeled using 3D motion graphics software (Autodesk Maya 2011).
• Micrograph of B. cenocepacia observed by contrast phase microscopy (magnification x 400). Overlay of 2D-DIGE gels (Cy2/Cy3/Cy5 minimal labeling dyes) scanned on a Typhoon Trio laser scanner. E-test plate for the determination of antimicrobial susceptibility. MALDI spectrum.
• Micrograph of B. cenocepacia observed by contrast phase microscopy (magnification x 400). 2D-DIGE gels (labeled using Cy2/Cy3/Cy5 minimal labeling dyes) scanned on a Typhoon Trio laser scanner and analyzed using Progenesis Samespots. E-test plate for the determination of antimicrobial susceptibility.
• Adhesion receptor-associated signalling complexes were isolated and resolved by polyacrylamide gel electrophoresis. Protein was visualised using Coomassie Blue and scanned using a flatbed scanner. Images were adjusted in Photoshop and compiled in Illustrator.
• Adhesion receptor-associated signalling complexes were isolated and analysed by mass spectrometry. An interaction network model of a specific adhesion complex was constructed using Cytoscape. Nodes were coloured according to relative enrichment over a related adhesion complex and rendered in Illustrator.
• Adhesion receptor-associated signalling complexes were isolated and analysed by mass spectrometry. The composition of different complexes was compared by agglomerative hierarchical clustering using Cluster 3.0 and displayed as a heat map using MultiExperiment Viewer.
• The composition of different adhesion receptor-associated signalling complexes, determined by mass spectrometry, was compared by hierarchical clustering (Cluster 3.0) and displayed as a heat map (Java TreeView). An interaction network model of the core receptor-bound subcomplex was constructed using Cytoscape.
• Pencil, scanner, Photoshop, protein structures from PDB, Molsoft ICM.
• Pencil, scanner, Photoshop, protein structures from PDB, Molsoft ICM.